Objective To evaluate the neuroprotective effects of lacosamide after experimental peripheral nerve injury in rats

Objective To evaluate the neuroprotective effects of lacosamide after experimental peripheral nerve injury in rats. for free oxygen radicals by increasing antioxidant enzyme activity. strong class=”kwd-title” Keywords: Lacosamide, Peripheral nerve injuries, Sciatic nerve INTRODUCTION Peripheral nerve injuries (PNI) can occur due to mechanical, chemical, and thermal reasons. Trauma is the most common reason and it is known that PNI occurs in 2.8% of trauma patients [19]. Although there are several drugs, steroids and hormones, whose positive effects have been reported clinically and experimentally, recovery after PNI is still a clinical challenge [2,14,21,24,29]. However, unlike in the central nervous system, regeneration in the peripheral anxious system can be done but full practical recovery is frequently poor [9]. It really is known that crush in peripheral anxious system leads to harm of intraneural microcirculation by immediate mechanical damage [16,31]. An inflammatory response amounts and strats of free of charge air radicals boost that leads to additional injury. Lipid peroxidation and the amount of cells malondialdehyde (MDA) also BFH772 raises which really is a poisonous procedure after PNI [12]. Consequently, many chemical substance real estate agents with antioxidant and antiinflammatory effect have been evaluated for their ability to inhibit this cascade [3-5, 11] Methylprednisolone and gabapentin are the reference agents and have shown beneficial effects in the literature [26]. But, although they have beneficial effects on parenchymal damage, there is no significant improvement BFH772 on functional recovery and there are several side effects associated with their use [7]. Lacosamide may be another pharmaceutical candidate for treatment after PNI. Although it is an antiepileptic drug in clinical use, it has been shown to protect neurological tissue and have Mouse monoclonal to Influenza A virus Nucleoprotein ameliorative effect on peripheral neuropathy. Several studies have reported that it exhibits antioxidant, anti-inflammatory and lipid peroxidation inhibiting effect which are the main scope of the treatment strategy for neural damage after PNI [1,13,22]. We have also reported in our previous study that lacosamide has neuroprotective effects after spinal cord injury [8]. So, when consider the anti-inflammatory, antioxidant, and inhibiting lipid peroxidation properties and protective effects of lacosamide on neural tissue after experimental spinal cord injury, we hypothesized that it may provide neuroprotective effects after traumatic PNI. To the best of our knowledge, no study has reported on the use of lacosamide in an experimental PNI model. The purpose of this study BFH772 was to investigate the effects of lacosamide after experimental sciatic nerve crush injury in rats using histopathological, biochemical and clinical methods. MATERIALS AND METHODS All the experimental procedures performed were approved by the Ethics Committee of Bingol University (date and serial number : 12.01.2017-796). A total of 28 male wistar albino rats weighing 300C350 g were divided into four groups of seven rats each. In group I (sham), the sciatic nerve exposed and the surgical wound was closed without injury; in group II, PNI was performed after dissection of the nerve; in group III, PNI was performed after dissection and lacosamide was administered, and, in group IV, PNI was performed after dissection and physiological saline solution was administered. All rats were kept under environmentally controlled conditions and housed in separate cages during the test and were fed standard rodent food and water. They were anesthetized by intraperitoneal injection of 10 mg/kg of xylazine (Alfazyne, Egevet, ?zmir, Turkey) and 50 mg/kg of ketamine (Ketalar, Parke-Davis, Eczac?ba??,.

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