Background: Extensive researches are going on to explore the effective and safe drug for their hair growth. were formulated by general method using o/w type base in various rations or concentrations such PF-3845 as 10% 20 and 30% of extract. These lotions were applied on shaved skin area of rats for 30 days once in a day and hair length serum total protein and total testosterone were measured. Results: Our formulations show increase in hair growth and serum total protein at concentration dependent manner with effect to standard and control groups. Serum total testosterone decreases according to a concentration dependent manner. Conclusion: Further series of investigations are however necessary to remain exploration which includes their structural elucidation characterization clinical safety reliability and molecular mechanism involved in this pharmacological activity. (Leguminosae) commonly known PF-3845 as LRRFIP1 antibody tobacco or in Hindi Tamakhu. Tobacco leaves posses narcotic sedative emetic carminative anthelmintic etc properties. The leaves also useful in the treatment of bronchitis asthma cancer of teeth skin diseases scorpion sting headache chronic giddiness and ranting.[20-24] The principal constituent of tobacco leaves is the alkaloid nicotine. They also contain a crystalline substance nicotianin and small quantities of alkaloids other than nicotine viz. nicotinine nicoteine and nicoteline together with traces of a volatile oil etc.[25] Hence the present study is an effort to formulate and evaluate the microbial biotransformed extract of tobacco leaves for hair growth potential selected on the basis of traditional use and evidence of microorganism responsible for biotransformation of leaves in cow urine. MATERIALS AND METHODS Plant material and extraction Fresh leaves of tobacco were collected in the month of August locally from the Nagpur. The plant and leaves were authenticated by a pharmacognocist Dr. Vinod D. Rangari Department of Pharmacognosy J. L. Chaturvedi College of Pharmacy Nagpur-440016 (MS) India. The leaves were dried under shade and macerated with cow urine for 28 days with occasional stirring. After filtration with muslin cloth solvent was removed by distillation under vacuum.[26] The crude residue mass of extract were concentrated stored and preserved (2-8° C). It is considered as a microbial biotransformed extract. Chemicals and reagents Minoxidil [Mintop 2 lotion] (Dr. Reddy’s Lab Hyderabad) and all other diagnostic kits and solvents used for experimental works were of AR grade. Animals Male albino wister rats (120-150 g) were used. The animals were fed with standard pellet diet and water and maintained under standard environmental conditions (22°C ± 5 °C with 12 h of light-dark cycle). All experimental protocols were approved by Institutional Animal Ethical Committee Clearance (JLCCP/IAEC 2007 J. L. Chaturvedi College of Pharmacy Nagpur-440016(M.S) India. Microbial study The isolation of microorganisms from the extract was done by using Streak Plate Technique.[27] These isolated microorganism culture were subjected to Disha Biotech Lab Nagpur for their identification having wide sample reference no. E07A187.02A (1141 and 1142) at dated 18 Oct 2007 Preparation of formulations Herbal lotions were prepared by general method using o/w type base. The formula of base contains lanoline (5% w/w) cetyl alcohol (3% w/w) bees wax (2% w/w) propylene glycol (1.5% w/w) sesame oil (1.5% w/w) stearic PF-3845 acid (2% w/w) preservative (q.s.) perfume (q.s.) is considered as phase-A commonly for all three lotion preparations (10% 20 and 30%). Phase-B was made by various concentrations of extract such as 10% w/w 20 w/w and 30% w/w by making volume upto 100 ml with the help of distilled water. After preparation of both phases phase-A was added slowly in phase-B by continuous triturating till uniform consistency of lotion was attained.[28] Hair growth activity The rats were divided into five groups of six rats each. A 4 cm PF-3845 2 area of dorsal portion of all rats were shaved and wiped with surgical spirit. Hair remover was also applied PF-3845 over the shaved area to assure the removal of trace of hairs. The animals in Group I considered as control Group II treated as standard. Applied 2% Minoxidil lotion over the shaved area once a day. Group III IV and V were considered as treatment groups Application of 10% 20 and 30% lotion formulations respectively. The treatment was continued for 30 days.[29] On day 15 and 30 of the treatment hairs were plucked randomly from the shaved area of selected rats and length of 24 hairs.