We present here the draft genome sequences of two colistin-resistant strains

We present here the draft genome sequences of two colistin-resistant strains belonging to sequence type 74 (ST74) and ST1850, isolated from commercial poultry meat in Brazil. performed using SPAdes 151038-96-9 version 3.9.0 (6). This assembly was curated using Geneious version R9 (Biomatters Ltd., New Zealand) and submitted for annotation using NCBI Prokaryotic Genome Annotation Pipeline version 3.2. Multilocus sequence types (MLST), plasmid replicons, antimicrobial resistance genes, and virulence genes were recognized using multiple databases: MLST 1.8, PlasmidFinder 1.3, ResFinder 2.1, and VirulenceFinder 1.5, respectively (http://genomicepidemiology.org/). CF111 and CF341 belonged to ST1850 and ST74, presenting 137 and 96 contigs distributed in genomes of 4,950,681?bp and 5,022,083?bp in size, respectively. In brief, CF111 offered 5,177 protein-coding genes, 55 RNA-coding genes (46 tRNAs, 2 rRNAs, and 7 noncoding RNAs [ncRNAs]), and 314 pseudogenes, with a G+C content of 50.7%, whereas CF341 presented 5,284 protein-coding genes, 64 RNA-coding genes (50 tRNAs, 1 rRNAs, and 13 noncoding RNAs [ncRNAs]), and 356 pseudogenes, with a G+C content of 50.6%. detection of plasmids recognized IncX4, IncFIB, and IncI1 in both isolates. On the other hand, IncFIC and IncFIA were recognized in CF111, whereas IncFII and IncFIB were recognized in CF341. In this regard, IncX4-type plasmids have been key vectors responsible for the dissemination of the gene in strains in food, humans, and animals in Brazil (3, 7, 8). In addition to the gene, while CF111 carried the -lactam resistance gene and CF341 harbored the -lactam resistance gene andaadA2and and in CF341 and and virulence genes 151038-96-9 in CF111. In summary, we statement the draft genome sequences of two colistin-resistant strains belonging to ST74 and ST1850, isolated in 2016 from commercial chicken meat in Brazil. Whole-genome sequence (WGS) analysis indicates that these strains carried the gene on IncX4-type plasmids, as previously reported in food, human, and animal strains from Brazil (3, 7, 8). These 151038-96-9 draft genome sequences could contribute to providing data to better understand the molecular mechanisms leading to the dissemination and successful circulation of strains in human, animal, and food production. Accession number(s). The genome sequences of strains CF111 and CF341 have been deposited at DDBJ/ENA/GenBank with accession figures “type”:”entrez-nucleotide”,”attrs”:”text”:”MUIP00000000″,”term_id”:”1141726149″,”term_text”:”MUIP00000000″MUIP00000000 and “type”:”entrez-nucleotide”,”attrs”:”text”:”MUIQ00000000″,”term_id”:”1141724268″,”term_text”:”MUIQ00000000″MUIQ00000000, respectively. ACKNOWLEDGMENTS This work was supported by research grants from Coordena??o de Aperfei?oamento de Pessoal de Nvel Superior (CAPES), Funda??o de Amparo Pesquisa do Estado de S?o Paulo (FAPESP), and Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (CNPq). N.L. is usually a research grant fellow of CNPq. We thank CEFAP-Genial facility for support for this investigation. Notes This paper was supported by the following grant(s): Funda??o de Amparo Pesquisa do Estado de S?o Paulo (FAPESP) 2016/03044-7 to . Funda??o de Amparo Pesquisa do Estado de S?o Paulo (FAPESP) 2013/07914-8 to . Funda??o de Amparo Pesquisa do Estado de S?o Paulo (FAPESP) 2016/08593-9 to . Footnotes Citation Monte DF, Fernandes MR, Cerdeira L, de Souza TA, Mem A, Franco BDGM, Landgraf M, Lincopan N. 2017. Draft genome sequences of 151038-96-9 colistin-resistant MCR-1-generating ST1850 and ST74 strains isolated from commercial chicken meat. Genome Announc 5:e00329-17. https://doi.org/10.1128/genomeA.00329-17. Recommendations 1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J. 2016. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a EXT1 microbiological and molecular biological study. Lancet Infect Dis 16:161C168. doi:10.1016/S1473-3099(15)00424-7. [PubMed] [Cross Ref] 2. Fernandes MR, Moura Q, Sartori L, Silva KC, Cunha MP, Esposito F, Lopes R, Otutumi LK, Gon?alves DD, Dropa M, Matt MH, Monte DF, 151038-96-9 Landgraf M, Francisco GR, Bueno MF, de Oliveira Garcia D, Kn?bl T, Moreno AM, Lincopan N. 2016. Silent dissemination of colistin-resistant in South America could contribute to the global spread of the mcr-1 gene. Euro Surveill 21:17. doi:10.2807/1560-7917.ES.2016.21.17.30214. [PubMed] [Cross Ref] 3. do Monte DF, Mem A, Fernandes MR, Cerdeira L, Esposito F, Galv?o JA, Franco BD, Lincopan N, Landgraf M. 2017. Chicken meat as reservoir of colistin-resistant transporting genes in South America [Epub ahead of print.] Antimicrob Brokers Chemother. doi:10.1128/AAC.02718-16. [PMC free article] [PubMed] [Cross Ref] 4..