Introduction Head and neck squamous cell carcinoma (HNSCC) is a common tumor worldwide and includes a poor prognosis. 0.05 were considered in multivariate Cox and analysis proportional hazards models. A Org 27569 two-sided worth significantly less than 0.05 was considered significant. Outcomes Patient features The 194 entitled sufferers with levels I-IVb HNSCC got a median age group of 54?years (range 25C86?years); 150 had been men (Desk?1). From the 194 sufferers, 156 had dental malignancies, 11 got supraglottic malignancies, and 21 got glottic malignancies. Adjuvant radiotherapy was presented with to 34 (17.5%) sufferers. The median follow-up period was 79?a few months (range 3C168?a few months). Just 14 (7.2%) sufferers were shed to follow-up. By the final follow-up visit, 116 (59.8%) patients had local or distant relapse events. The disease-specific mortality was 41.8% (81/194). Table?1 Associations between T lymphoma invasion and metastasis 1 (Tiam1) expression and clinicopathologic characteristics of the 194 patients with head and neck squamous Org 27569 cell carcinoma (HNSCC) Cut-off score selection The ROC curve for the scores of Tiam1 expression was plotted to select the appropriate cut-off score (Fig.?1). The area under the curve was 0.692 [P?0.001, 95% confidence interval (CI) 0.615C0.768]. A Tiam1 score of 5 maximized the Youden Index [sensitivity (0.802)?+?specificity (0.584)???1?=?0.386] as the optimal cut-off Org 27569 score. Thus, cases were divided into low (score?5) and high (score??5) expression groups. Fig.?1 Selection of the cut-off score for T lymphoma invasion and metastasis 1 (Tiam1) expression by the receiver operating characteristic (ROC) analysis. The area under the curve (AUC) is usually 0.692. An expression score of 5 maximizes the Youden Index [sensitivity ... Tiam1 expression and clinicopathologic factors The Tiam1 protein was located in the cytoplasm (Fig.?2). The Org 27569 high expression rate was higher in tumor tissues than in the matched noncancerous tissues (57.7% vs. 13.9%, P?0.001). High Tiam1 expression was significantly associated with relapse (P?0.001), lymph node metastasis (P?=?0.003), and stage III/IV cancers (P?0.001) (Table?1). The association between Tiam1 expression and tumor position was marginally significant (P?=?0.064). Fig.?2 Tiam1 expression in tumor samples Mouse monoclonal to TEC and noncancerous tissue samples. Tiam1 protein staining mainly distributes in the cytoplasm. a (100) and b (200), strong staining of Tiam1 protein in tumor cells. c (100) and d (200), … Predictive value of the Tiam1 expression score The 1-, 3-, and 5-12 months OS rates of all patients were 91.8, 70.5, Org 27569 and 64.7%, respectively. The 1-, 3-, and 5-12 months DFS rates were 64.8%, 48.1%, 43.2%, respectively. The relapse rate was higher in the high Tiam1 expression group than in the low Tiam1 expression group (70.5% vs. 45.1%, P?0.001), as was the mortality (58.0% vs. 19.5%, P?0.001). In the high Tiam1 expression group, the median OS was 61.5?months (95% CI 41.3C81.7?months), and the median DFS was 15.2?months (95% CI 6.7C23.7?months). The 5-12 months OS and DFS rates were 51.8% and 31.0%, respectively. The low Tiam1 expression group had a rather improved long-term survival: the 5-12 months OS and DFS rates were 82.7% and 60.0%, respectively. High Tiam1 expression predicted short OS and DFS (both P?0.001, Fig.?3). In univariate survival analysis, patients using tobacco and alcohol with poorly differentiated tumors, lymph node metastasis, and stage III or IV disease had shorter OS survival, and patients with poorly differentiated tumors, no neck dissection, advanced disease, and oral cavity cancer had shorter DFS than their counterparts (Table?2). Cox proportional hazards analysis revealed that high Tiam1 expression independently predicted short OS.